TABLE 1.
Effect of methylamine, ethylamine, and bafilomycin A1 on rounding activity of iota-toxinlegend
| Treatment | Effect of dose of Ia (ng/ml)
|
||||
|---|---|---|---|---|---|
| 1 | 5 | 10 | 50 | 100 | |
| None | − | + | ++ | +++ | +++ |
| Methylamine | − | − | − | − | + |
| Ethylamine | − | − | − | − | + |
| Bafilomycin A1 | − | − | − | − | − |
legend
Vero cells were preincubated with methylamine (20 mM), ethylamine (20 mM), or bafilomycin A1 (500 nM) at 37°C for 30 min. The cells were then incubated with various amounts of Ia in the presence of Ib (50 ng/ml). Cell rounding was recorded after 8 h. Cell rounding in a Vero cell assay (cytotoxic activity) was scored as follows: +++, 100% rounding; ++, 50 to 80% rounding; +, 20 to 40% rounding and −, no rounding.