TABLE 1.
Oligonucleotides
| Oligonucleotide | Sequencea | Description |
|---|---|---|
| N32 | 5′-ATGGCAGGAAACACAATTGGA-3′ | 5′ primer for aroC |
| N33 | 5′-CCAGCGTGGAATCTCTGTCTT-3′ | 3′ primer for aroC |
| Tet1 | 5′-GAagatctGGCCCTTTCGTCTTCACCTC-3′ | 5′ primer for tetR |
| Tet2 | 5′-CTAgctagcTAGCTCCTGAAAATCTCGCC-3′ | 3′ primer for tetR |
| Neo1 | 5′-CTAgctagcATAACTTCGTATAGCATACATTATACGAAGTTATCTTCACGCTGCCGCAAGCAC-3′ | 5′ primer for neo |
| Neo2 | 5′-CTAgctagcATAACTTCGTATAGCATACATTATACGAAGTTATGACCAAAGCGGCCATCGTGC-3′ | 3′ primer for neo |
| Cre1 | 5′-GGggtaccATGTCCAATTTACTGACCG-3′ | 5′ primer for cre |
| Cre2 | 5′-AActgcagCTAATCGCCATCTTCCAGC-3′ | 3′ primer for cre |
| Luci1 | 5′-GGggtaccATGGAAGACGCCAAAAACAT-3′ | 5′ primer for luci |
| Luci2 | 5′-AActgcagTTACAATTTGGACTTTCCGC-3′ | 3′ primer for luci |
a
Endonuclease sites are in lowercase letters, and the loxP sequence is underlined.