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SEB movement across epithelial cell monolayers. SEB (10, 50, 100, or 300 μg/ml) was added to the apical or basal side of confluent T-84 or Caco-2 cell monolayers. The opposing chamber was sampled over a 24-h period, and the amount of toxin present was determined by sandwich ELISA. Apical to basal movement is depicted as a solid line, and basal to apical movement is depicted as a dashed line. Each point represents the mean of three replicate experiments ± standard error.
