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. 2002 Apr;70(4):2039–2048. doi: 10.1128/IAI.70.4.2039-2048.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 3. — Proliferative responses of T-cell lines (CL) from the B. bovis-immune cattle C97, C15, and G3 against synthetic peptides (Table 2) spanning the RAP-1 NT region. Short-term T-cell lines from cows C97, C15, and G3 were stimulated with 1 and 10 μg of peptides P1 to P9/ml. As a negative control, a 30-mer peptide derived from A. marginale MSP-2 was used. Results are the mean counts per minute of triplicate cultures of T cells stimulated with antigen for 3 days ± 1 standard deviation. Responses significantly higher than those for A. marginale MSP-2 peptide are indicated with a † (P < 0.005) or ∗ (P < 0.05).

FIG. 3. — Proliferative responses of T-cell lines (CL) from the B. bovis-immune cattle C97, C15, and G3 against synthetic peptides (Table 2) spanning the RAP-1 NT region. Short-term T-cell lines from cows C97, C15, and G3 were stimulated with 1 and 10 μg of peptides P1 to P9/ml. As a negative control, a 30-mer peptide derived from A. marginale MSP-2 was used. Results are the mean counts per minute of triplicate cultures of T cells stimulated with antigen for 3 days ± 1 standard deviation. Responses significantly higher than those for A. marginale MSP-2 peptide are indicated with a † (P < 0.005) or ∗ (P < 0.05).