FIG. 4.
Effect of exogenous IFN-γ on expression of IL-10 mRNA in THP-1 cells stimulated with L-OspA (A) and LPS (B). Vitamin D3-treated THP-1 cells (106/ml) were incubated with L-OspA (1 μg/ml) or LPS (1 μg/ml) in the presence (black bars) or absence (white bars) of 10 ng of human rIFN-γ per ml. At different times p.s., the mRNA levels of IL-10 were determined by RT-PCR. Responses are shown as fold increase over the level in unstimulated cells. All values were normalized with respect to GAPDH mRNA levels. Results represent the mean value of two determinations (each from a separate RNA batch) ± standard error.