FIG. 4.

Western blot analysis of T. denticola HL201 pKMCouflgE transformants. Cell extracts were separated on a sodium dodecyl sulfate-4 to 20% polyacrylamide gel electrophoresis gel and transferred to a Hybond C Extra membrane. Antibody developed to the T. pallidum FlgE protein was used as the primary antibody (1:3,000 dilution), and horseradish peroxidase-conjugated goat anti-mouse IgG (1:250,000 dilution) was used as the secondary antibody (Pierce, Rockford, Ill.). The blot was developed by using Supersignal West Dura Western blotting kits (Pierce). Lane 1, cell extract of wild-type T. denticola 33520; lane 2, cell extract of T. denticola flgE mutant HL201; lane 3, cell extract of T. denticola HL201 pKMCouflgE transformants. Numbers on the left indicate molecular masses in kilodaltons.