FIG. 1.

Induction of DTH by cryptococcal supernatants (sups). Mice were immunized with 280 μg of lyophilized supernatants from the A9759 C. neoformans strain (A9759 sups) (A) or with B. dermatitidis (10⁷ cells) (B). Control nonimmunized mice received PBS only. Seven days later, mice were challenged in the right hind pad by the inoculation of A9759 or Cap-67 supernatants (35 μg) or B. dermatitidis (5 × 10⁴ cells). Results indicate differences in weight between the right hind pad (challenged with the test preparation) and the left one (inoculated with vehicle only). Data represent means ± standard deviations of six observations conducted during three separate experiments. ∗, P < 0.05, relative to nonimmunized mice, by one-way analysis of variance and the Student-Keuls-Newman test.