TABLE 2.
Expression of Th1-like cytokines from stimulated splenocytes obtained from B. mallei-vaccinated BALB/c mice
| Cytokine | Group vaccinateda | Amt of cytokine expressed (pg/ml)b in:
|
|||
|---|---|---|---|---|---|
| Medium | Medium with stimulating antigen
|
||||
| Cellc | ConA | LPS | |||
| IL-2 | HKGB15 | 24 ± 5 | 174 ± 41* | 6,245 ± 2,411 | 27 ± 6 |
| IRGB15 | 26 ± 6 | 166 ± 26* | 8,657 ± 2,653 | 28 ± 7 | |
| CapGB15 | 26 ± 6 | 215 ± 81* | 9,833 ± 1,515 | 21 ± 3 | |
| Adjuvant | 19 ± 2 | 28 ± 5 (HK) | 8,118 ± 2,343 | 19 ± 2 | |
| Adjuvant | 19 ± 2 | 34 ± 2 (IR) | 8,118 ± 2,343 | 19 ± 2 | |
| Adjuvant | 19 ± 2 | 29 ± 5 (Cap) | 8,118 ± 2,343 | 19 ± 2 | |
| IL-12 | HKGB15 | 206 ± 18 | 330 ± 48 | 561 ± 56 | 295 ± 29 |
| IRGB15 | 239 ± 37 | 491 ± 61 | 443 ± 115 | 241 ± 13 | |
| CapGB15 | 233 ± 26 | 623 ± 85 | 556 ± 131 | 311 ± 139 | |
| Adjuvant | 207 ± 13 | 322 ± 40 (HK) | 419 ± 48 | 263 ± 13 | |
| Adjuvant | 207 ± 13 | 432 ± 26 (IR) | 419 ± 48 | 263 ± 13 | |
| Adjuvant | 207 ± 13 | 763 ± 102 (Cap) | 419 ± 48 | 263 ± 13 | |
| IFN-γ | HKGB15 | 3,576 ± 298 | 45,452 ± 6,035* | 43,047 ± 11,400 | 42,993 ± 5,886 |
| IRGB15 | 3,874 ± 223 | 48,507 ± 16,541* | 36,361 ± 13,700 | 56,778 ± 7,525 | |
| CapGB15 | 3,474 ± 372 | 59,907 ± 11,176* | 58,193 ± 13,114 | 41,503 ± 5,886 | |
| Adjuvant | 3,129 ± 372 | 18,702 ± 3,949 (HK) | 32,263 ± 6,035 | 47,240 ± 9,612 | |
| Adjuvant | 3,129 ± 372 | 31,742 ± 3,353 (IR) | 32,263 ± 6,035 | 47,240 ± 9,612 | |
| Adjuvant | 3,129 ± 372 | 32,412 ± 6,482 (Cap) | 32,263 ± 6,035 | 47,240 ± 9,612 | |
a
There were four mice per group, and spleens were processed as described in Materials and Methods.
b
Results are means ± SDs. *, P < 0.01 for comparison with the homologous adjuvant control.
c
“Cell” refers to the homologous stimulating antigen. Abbreviations in parentheses represent stimulating antigens: HK, heat-killed GB15; IR, irradiation inactivated GB15; Cap, capsule-negative GB15.