TABLE 3.
Expression of Th2-like cytokines from stimulated splenocytes obtained from B. mallei-vaccinated BALB/c mice
| Cytokine | Group vaccinateda | Amt of cytokine expressed (pg/ml)b in:
|
|||
|---|---|---|---|---|---|
| Medium | Medium with stimulating antigen
|
||||
| Cellc | ConA | LPS | |||
| IL-4 | HKGB15 | 30 ± 2 | 58 ± 15** | 586 ± 131 | 30 ± 7 |
| IRGB15 | 27 ± 2 | 49 ± 6** | 696 ± 270 | 25 ± 2 | |
| CapGB15 | 24 ± 4 | 47 ± 18* | 618 ± 324 | 23 ± 3 | |
| Adjuvant | 20 ± 1 | 21 ± 2 (HK) | 470 ± 148 | 25 ± 2 | |
| Adjuvant | 20 ± 1 | 34 ± 2 (IR) | 470 ± 148 | 25 ± 2 | |
| Adjuvant | 20 ± 1 | 29 ± 5 (Cap) | 470 ± 148 | 25 ± 2 | |
| IL-5 | HKGB15 | 29 ± 2 | 44 ± 15** | 120 ± 21 | 44 ± 24 |
| IRGB15 | 40 ± 20 | 105 ± 40 | 53 ± 31 | 29 ± 2 | |
| CapGB15 | 29 ± 9 | 35 ± 7** | 88 ± 38 | 26 ± 4 | |
| Adjuvant | 25 ± 2 | 27 ± 4 (HK) | 89 ± 29 | 30 ± 5 | |
| Adjuvant | 25 ± 2 | 83 ± 66 (IR) | 89 ± 29 | 30 ± 5 | |
| Adjuvant | 25 ± 2 | 24 ± 3 (Cap) | 89 ± 29 | 30 ± 5 | |
| IL-10 | HKGB15 | 76 ± 7 | 483 ± 94** | 491 ± 111 | 556 ± 163 |
| IRGB15 | 73 ± 7 | 613 ± 122** | 519 ± 99 | 609 ± 116 | |
| CapGB15 | 88 ± 13 | 1,175 ± 859** | 449 ± 169 | 510 ± 165 | |
| Adjuvant | 80 ± 21 | 210 ± 32 (HK) | 342 ± 48 | 283 ± 79 | |
| Adjuvant | 80 ± 21 | 375 ± 43 (IR) | 342 ± 48 | 283 ± 79 | |
| Adjuvant | 80 ± 21 | 300 ± 69 (Cap) | 342 ± 48 | 283 ± 79 | |
a
There were four mice per group, and spleens were processed as described in Materials and Methods.
b
Results are means ± SDs. Statistical significance: * or **, P < 0.05 or P < 0.01, respectively, for comparison with the homologous adjuvant control.
c
“Cell” refers to the homologous stimulating antigen. Abbreviations in parentheses represent stimulating antigens: HK, heat-killed GB15; IR, irradiation inactivated GB15; Cap, capsule-negative GB15.