TABLE 4.
Effect of adjuvant on ratio of IgG2a to IgG1 in response to B. mallei immunization in BALB/c mice
| Expt | Treatmenta | Immunogenb | Titer (102)c of:
|
Ratio of titers (IgG2a/IgG1) | |
|---|---|---|---|---|---|
| IgG1 | IgG2 | ||||
| I | With adjuvant (100 μg) | HKGB15 | 2,400 ± 924 | 575 ± 695 | 0.24 |
| IRGB15 | 12,800 ± 9,050 | 1,800 ± 1,007 | 0.14 | ||
| CapGB15 | 3,800 ± 3,020 | 1,900 ± 9 | 0.50 | ||
| II | Without adjuvant (50 μg) | HKGB15 | 160 ± 113 | 44 ± 24 | 0.28 |
| IRGB15 | 2,330 ± 2,080 | 960 ± 370 | 0.41 | ||
| CapGB15 | 2,880 ± 4,909 | 1,387 ± 1,124 | 0.48 | ||
a
The amount of adjuvant used in the study is described in Materials and Methods. The number in parentheses represents the amount of each immunogen used per immunization.
b
The mice (four per group) were immunized twice, and sera were collected 21 days after the second booster.
c
The Ig titers for mice treated with adjuvant only (experiment I) or PBS only (experiment II) were both less than 50, as determined with IRGB15 cells. Results are reported as means ± SD.