TABLE 7.
Ig class and IgG1 and IgG2a subclass response in surviving B. mallei-immunized BALB/c mice after parenteral challenge
| Immunogena | No. of survivorsb | Titer (103)c of:
|
Ratio of titers (IgG2a/IgG1) | |||
|---|---|---|---|---|---|---|
| IgG | IgM | IgG1 | IgG2a | |||
| HKGB15 | 4 | 320 ± 3.2 | 40 ± 0.2 | 320 ± 4.5 | 160 ± 3.0 | 0.5 |
| IRGB15 | 5 | 320 ± 3.2 | 40 ± 0.3 | 1,280 ± 23 | 1,280 ± 14 | 1.0 |
| CapGB15 | 5 | 640 ± 3.2 | 80 ± 0.4 | 2,560 ± 51 | 640 ± 4.5 | 0.25 |
| Adjuvant | 2 | 32 ± 0.3 | 32 ± 0.2 | 16 ± 0.2 | 64 ± 0.9 | 4.0 |
a
Two immunizations were given per mouse as stated in Materials and Methods. All mice (n = 5 per group) were challenged i.p. with 2.4 × 107 cells of GB15.1-2 4 weeks after the second immunization.
b
Mice were observed for 21 days after the challenge.
c
Sera were collected from the survivors and pooled, and titers were determined in triplicate and reported as means ± SDs. The titer for the group treated with adjuvant only was determined with IRGB15 cells.