Skip to main content
. 2002 May;70(5):2535–2543. doi: 10.1128/IAI.70.5.2535-2543.2002

FIG. 3.

FIG. 3.

Brucella Cu,Zn SOD-specific antibody isotype responses stimulated by O. anthropi strain 49237SOD with (hatched bars)or without (solid bars) the CpG DNA adjuvant. Groups of five mice were immunized i.p. with O. anthropi strain 49237SOD with or without the CpG DNA adjuvant. Mice were bled individually at 5 weeks after immunization, and their serum samples were used at a 1:100 dilution for detection of IgG (A), IgG1 (B), and IgG2a (C) responses to purified Brucella Cu,Zn SOD by indirect ELISA. Each serum sample was tested in duplicate. A significant difference was shown only in the production level of IgG1 between vaccinations with O. anthropi strain 49237SOD alone and O. anthropi strain 49237SOD plus CpG-ODN (B) (P < 0.01).