TABLE 2.
Induction of protective immunity against B. abortus 2308 challenge by immunization with O. anthropi 49237 or 49237SOD in combination with CpG-ODN
| Group | Vaccine (dose) | Log10 CFU in spleens (x ± SD) | P |
|---|---|---|---|
| Experiment A | |||
| 1 | Saline | 5.31 ± 0.16 | Control |
| 2 | CpG-ODN alone | 5.33 ± 0.21 | >0.05 |
| 3 | E. coli (1 × 106) + CpG-ODN | 5.28 ± 0.18 | >0.05 |
| 4 | O. anthropi 49237 (5 × 108) alone | 5.25 ± 0.18 | >0.05 |
| 5 | O. anthropi 49237 (5 × 108) + CpG-ODN | 4.04 ± 0.95 | <0.05*a |
| 6 | O. anthropi 49237SOD (5 × 108) | 5.25 ± 0.21 | >0.05 |
| 7 | O. anthropi 49237SOD (5 × 108) + CpG-ODN | 2.30 ± 1.01 | <0.01** |
| Experiment B | |||
| 1 | Saline | 5.69 ± 0.14 | Control |
| 2 | CpG-ODN alone | 5.49 ± 0.33 | >0.05 |
| 3 | O. anthropi 49237SOD (5 × 108) | 5.24 ± 0.49 | >0.05 |
| 4 | O. anthropi 49237SOD (5 × 108) + CpG-ODN | 3.73 ± 1.22 | <0.01** |
| Experiment C | |||
| 1 | Saline | 5.30 ± 0.12 | Control |
| 2 | CpG-ODN alone | 5.28 ± 0.15 | >0.05 |
| 3 | O. anthropi 49237 (5 × 108) alone | 5.25 ± 0.15 | >0.05 |
| 4 | O. anthropi 49237SOD (5 × 108) | 5.25 ± 0.15 | >0.05 |
| 5 | O. anthropi 49237SOD (5 × 108) + CpG-ODN | 2.88 ± 1.23 | <0.01** |
a
* and **, significantly different from the saline group and each other.