TABLE 3.
Clearance of O. anthropi strains 49237, 49237pBB, and 49237SOD with or without CpG adjuvant from mouse spleens
| No. of days postinoculation | Mean log10 CFU/spleen ± SDa in mice inoculatedb with:
|
||||
|---|---|---|---|---|---|
| 49237pBB | 49237 | 49237 + CpG | 49237SOD | 49237SOD + CpG | |
| 5 | 4.08 ± 0.36 | 3.27 ± 0.36 | 3.59 ± 0.26 | 4.91 ± 0.33 | 4.74 ± 0.51 |
| 8 | 2.06 ± 0.49 | 2.01 ± 0.24 | 2.11 ± 0.22 | 2.89 ± 0.26 | 2.70 ± 0.35 |
| 14 | —c | — | — | — | — |
No significant difference was found between O. anthropi strain 49237 alone and 49237 plus CpG-ODN (P > 0.05) or between O. anthropi strain 49237SOD alone and strain 49237SOD plus CpG-ODN (P > 0.05) at 5 or 8 days postinoculation. However, strain 49237 (or 49237 plus CpG-ODN) had significantly lower CFU than strain 49237SOD (or 49237SOD plus CpG-ODN) at 5 or 8 days postinoculation (P < 0.05). Clearance of strain 49237pBB was significantly different from that for all the other groups at 5 days postinoculation (P < 0.05) but different only from strain 49237SOD alone and strain 49237SOD plus CpG-ODN (P < 0.05) at 8 days postinoculation.
Inoculation doses, 3.6 × 108 (log10 8.56) for O. anthropi 49237 and 49237pBB; 2.2 × 108 (log10 8.34) for O. anthropi 49237SOD. Each of five mice per group were inoculated i.p.
—, undetectable level (detection limit, 20 CFU/spleen, or log10 1.3 CFU/spleen).