TABLE 3.
Distribution of O-antigen ORFs of PPD134/91 among various A. hydrophila strains
| Strain | Sero- group | O-antigen ORF of A. hydrophila PPD134/91a
|
||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | ||
| ATCC 7966 | O:1 | (+) | − | (+) | (+) | (+) | − | − | − | − | − | − | (+) | (+) | (+) | (+) | − | − |
| JCM 3968 | O:6 | + | − | (+) | (+) | (+) | − | − | − | − | − | + | (+) | (+) | (+) | (+) | − | − |
| JCM3973 | O:11 | + | − | (+) | (+) | − | − | (+) | − | − | − | (+) | (+) | − | (+) | (+) | − | + |
| JCM3976 | O:14 | + | − | (+) | (+) | − | − | − | − | − | − | − | − | − | (+) | (+) | − | − |
| JCM3978 | O:16 | + | + | (+) | (+) | (+) | − | (+) | − | − | (+) | − | − | − | (+) | (+) | − | (+) |
| JCM3980 | O:18 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| JCM3981 | O:19 | (+) | − | (+) | (+) | (+) | − | (+) | − | − | − | − | − | − | (+) | (+) | − | − |
| JCM3983 | O:21 | + | − | (+) | (+) | (+) | − | (+) | − | − | − | − | − | − | (+) | − | − | − |
| JCM3984 | O:22 | + | − | (+) | (+) | (+) | − | − | − | − | − | − | (+) | (+) | (+) | (+) | − | − |
| JCM3985 | O:23 | − | − | (+) | (+) | (+) | − | − | − | − | + | − | (+) | (+) | (+) | − | − | (+) |
| JCM3996 | O:34 | + | + | + | + | + | (+) | (+) | (+) | + | − | + | + | + | + | + | + | + |
| L15 | Unknown | − | − | (+) | (+) | − | − | − | − | − | − | − | − | − | − | − | − | − |
| L31 | Unknown | + | − | − | (+) | − | − | − | − | − | − | − | − | − | − | − | − | − |
| L36 | Unknown | − | − | (+) | (+) | − | − | − | − | − | − | − | − | − | − | − | − | − |
| PPD35/85 | Unknown | − | − | (+) | (+) | − | − | (+) | − | − | − | − | − | − | − | − | − | − |
| PPD11/90 | Unknown | + | − | (+) | (+) | − | − | (+) | − | − | − | − | (+) | (+) | (+) | − | − | − |
| PPD64/90 | Unknown | + | + | + | + | + | (+) | (+) | (+) | (+) | (+) | + | + | + | + | + | + | + |
| PPD88/90 | Unknown | + | − | (+) | (+) | (+) | − | (+) | − | − | (+) | − | − | (+) | (+) | − | − | (+) |
| PPD45/91 | Unknown | + | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − | − |
| PPD70/91 | Unknown | + | − | (+) | (+) | − | − | (+) | − | − | − | − | − | (+) | (+) | − | − | − |
| PPD122/91 | Unknown | + | − | (+) | (+) | (+) | − | (+) | − | − | − | (+) | − | − | (+) | − | − | − |
| PPD134/91 | O:18 | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + | + |
| TF7 | O:11 | (+) | − | (+) | (+) | (+) | − | (+) | − | − | − | − | − | − | (+) | (+) | − | − |
a
Internal primers were designed for the 17 O-antigen ORFs. PCR was performed using genomic DNAs from different A. hydrophila strains as templates in order to survey their distribution among these strains. Those samples which had only one band of the same size as the coding gene in PPD134/91 were taken as positive. Southern hybridization was also employed to confirm the distributions of the 17 O-antigen ORFs among these strains. Symbols: +, positive; −, negative; (+), strains which were negative in PCR results but positive in Southern hybridization analysis.