FIG. 5.

Level of apoptosis in the genital tracts of wild-type and IFN-γ-deficient mice infected with Chlamydia. Mice were infected with C. muridarum for 7 days, histological samples were stained by TUNEL, and the number of apoptotic cells in the uterine horns and oviducts was determined as described in Materials and Methods. All apoptosis values were normalized to the value obtained with infected IFN-γ-deficient (IFNγ KO) mice, which was defined as 100. Histological sections were prepared from three uninfected mice and three infected mice from each group.