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. 2002 May;70(5):2566–2575. doi: 10.1128/IAI.70.5.2566-2575.2002

FIG. 2.

FIG. 2.

MAb 24c5 ELISA analysis and binding to glycogen. (A) Splenic fusion resulted in MAb 24c5, an IgG1 able to recognize M. tuberculosis GC. Each data point represents the average of three measurements. (B) MAb 24c5 binding to dilute concentrations of GC in the M. tuberculosis GC ELISA. Each data point represents the average of three measurements. (C) MAb 24c5 binding to M. tuberculosis Erdman day-20 7H9 medium (MTB), cell culture supernatant extract (INTPHSE), GC, AM, lipoarabinomannan (LAM), phosphatidylinositol mannoside (PIM), fast-growing mycobacterial lipomannan (LM), mycolyl-arabinogalactan-peptidoglycan complex (mAGP), and total lipid fraction (TLF). Each bar represents the average of two measurements. (D) Effects of proteinase K on binding of MAb 24c5 to M. tuberculosis Erdman day-20 7H9 medium. (E and F) MAb 24c5 assayed for reactivity to type VIII (slipper limpet) (viii) (E) and type IX (ix) (bovine liver) (F) glycogens. Each data point represents the average of three measurements. Error bars show the standard deviations of the means.