FIG. 4.

IF microscopy of M. tuberculosis Erdman using MAb 24c5. Formalin-fixed M. tuberculosis Erdman grown in the absence of Tween for 25 days was analyzed by IF microscopy using ALEXA-488-conjugated MAb 24c5 (A and B) or irrelevant control MAb (C). Bars = 5 μm. Slides were viewed at a magnification of ×100 using oil immersion. For photography an exposure time of 27 s was used for all samples. Slides were scanned into Adobe Photoshop, version 5.0, and images were transferred to Microsoft Powerpoint, version 2000, without any modification except enlargement.