TABLE 1.

BFTs purified by Van Tassell and urea-based methods

Method and protein	Total protein (μg) (mean ± SEM)	Total activity (U) (mean ± SEM)	Sp act (U/μg) (mean ± SEM)	% Activity recovered (mean ± SEM)	Final titer (ng/ml) (mean ± SEM)
Van Tassell
BFT-1 (n = 5)
Culture supernatant	9.93 (±0.66) × 106	23.10 (±7.34) × 106	2.39 (±0.8)
Mono Q fraction	22.44 (±5.29)	3.23 (±0.67) × 106	1.49 (±0.20) × 105	17 (±2.57)	0.37 ± 0.06
BFT-2 (n = 5)
Culture supernatant	10.55 (±1.23) × 106	48.19 (±1.81) × 106	4.75 (±0.4)
Mono Q fraction	26.02 (±3.32)	10.83 (±1.72) × 106	4.34 (±0.65) × 105	23 (±4.11)	0.13 ± 0.02
Urea based
BFT-2 (n = 3)
Culture supernatant	8.46 (±1.45) × 106	53.33 (±10.67) × 106	6.23 (±0.24)
Urea Mono Q fraction	77.31 (±29.14)	23.39 (±1.38) × 106	3.24 (±0.45) × 105	44.0 (±10.44)	0.07 ± 0.02