FIG. 4.

M. avium subsp. paratuberculosis antisense FAP-P mutants are inefficiently attached and internalized by epithelial cells. M. avium subsp. paratuberculosis strains containing pTS026 (26A), pTS028 (28F and 28H), or the vector control pWES4 (W4C) were incubated with FN and used to infect T-24 (open bars) and Caco-2 (filled bars) cells. The data from three independent experiments were combined and compared by Student's t test. A single asterisk indicates a P value of <0.05; a pair of asterisks indicates a P value of <0.01. (A) Total number of cells binding mycobacteria; (B) total number of cells ingesting mycobacteria.