TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | endA1 hsdR17 supE44 ΔlacU169(φ80lacZDM15) recA1 gyrA96 thi-1 relA1 | Boehringer |
| XL1-BlueMRF | Δ(mcrA) 183 Δ(mcrCB-hsdSMR-mrr) 173 endA1 supE44 thi-1 recA1 gyrA96 relA1 lac [F′proAB lacIq ZΔM15 Tn10 (Tet1)] | Stratagene |
| EC 101 | E. coli JM101 derivative with repA from pWV01 integrated into the chromosome | 16 |
| S. agalactiae | ||
| O90R (ATCC 12386) | R. Lancefield grouping strain, Hly+ | American Type Culture Collection |
| RGFB-K1 | O90R derivative, rgfB::pGh9:ISS1 | This study |
| RGFC-K2 | O90R derivative, rgfC::pGh5 | This study |
| Plasmids | ||
| pGh9:ISS 1 | Eryr, ori Ts | 17 |
| pGh5 | Eryr, ori pBR, ori Ts | Appligene |
| pBS 1966 | pGh5 derivative carrying an internal 469-bp fragment of rgfC | This study |