TABLE 1.
Summary of DT binding affinity and the number of DT binding sites per cell
| Cell linea | Kd (nM)b | n (105)b |
|---|---|---|
| LCD9/Mk HB-EGF | 7.0 | 8.7 |
| LCD9/F115A | 20.0 | 5.4 |
| LCD9/I117A | 4.0 | 1.7 |
| LCD9/Y138A | 6.6 | 6.5 |
| LCD9/L148A | 3.6 | 2.4 |
| LCD9/F115A/L148A | 15.9 | 5 |
| LCD9/I117A/L148A | 3 | 4.6 |
| LCD9/Y138A/L148A | 6.8 | 11.8 |
a
These cell lines are LCD9 cells expressing wild-type or mutant Mk proHB-EGF/DT receptors.
b
All binding assays were done in duplicate and were performed at least twice for each cell line with the average calculated Kd and number of binding sites per cell (n) being reported.