TABLE 2.
Interaction of DT with immobilized HB-EGF moleculesa
| Recombinant HB-EGF protein used | Avg Ka (104) (1/Ms) | Avg Kd (10−3) (1/S) | Avg KD (10−8) (M) |
|---|---|---|---|
| Mature Hu HB-EGF | 4.6 | 1.8 | 3.8 |
| Mature Mk HB-EGF | 6.4 | 2.2 | 3.4 |
| HBΔ-EGF | 4.9 | 2.5 | 5.2 |
| HBΔ-EGF(I117A/L148A) | 7.1 | 7.1 | 10 |
a
The BIAcore 2000 system with CM5 sensor chips was employed. The recombinant HB-EGF proteins were immobilized on CM5 sensor chips by standard amine coupling, and the kinetic experiments were performed as described in Materials and Methods. This table shows values for a representative experiment of two separate experiments.