Fig. 6.

Effect of erastin treatment on reactive oxygen species (ROS) levels in Cryptocaryon irritans tomonts under normoxia and hypoxia (n = 3). a Fluorescence intensity at E_x/E_m = 488/525 nm. b ROS fluorescence staining images of C. irritans tomonts under E_x/E_m = 488/525 nm at 48 h in each group. Scale bar, 100 µm. ROS at 24 h were analyzed using the Kruskal–Wallis test followed by Dunn’s multiple-comparisons test, whereas ROS at 48 h were analyzed by one-way ANOVA with Dunnett’s multiple comparisons test. Data are shown as mean ± SD. ‘n’ indicates biological replicates. *p < 0.05 and **p < 0.01