FIG. 2.

Expression of recombinant TibC protein in E. coli BL21(DE3). Total cellular proteins of different recombinant E. coli strains were separated by SDS-10% PAGE. The plasmids contain a tibC gene derived from the particular ETEC strain indicated in the plasmid name. Proteins were detected by staining with Coomassie blue. The total cellular extracts were prepared from recombinant bacteria without (−) and with (+) prior induction of T7 polymerase synthesis with IPTG for 3 h. Molecular sizes of the marker proteins are given in kilodaltons.