FIG. 5.

Detection of transcripts by RT-PCR. RNA was extracted from strain D39 in the nasal washes (a) or from strain EF3030 in the nasal washes and lung homogenates (b) of CBA/N mice. RNA was treated with DNase I. Transcripts were detected by RT-PCR with gene-specific primers. Transcripts detected were for pspC, endA, and pspA (a) and pspC (b). The plus and minus signs in panel b indicate reactions carried out in the presence (test) or absence (control) of RT, respectively. Similar controls were also used for the experiments whose results are shown in panel a.