TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmid	Description	Reference or source
H. influenzae biogroup aegyptiusa
F3031	Invasive BPF isolate, prototype strain	7
F1947	Noninvasive non-BPF isolate	7
F3029	Invasive BPF isolate	7
F3033	Invasive BPF isolate	7
F3037	Invasive BPF isolate	7
F4380	Invasive BPF isolate from Australia	37
Connecticut	Invasive BPF isolate from United States	CDCb via A. Lesse
Valparaiso	Invasive BPF isolate from Brazil	CDC via A. Lesse
H. influenzae
DL42	Type b	E. Hansen
DL63	Type b	E. Hansen
Eagan	Type b	S. Goodgal
TN106	Nontypeable	E. Hansen
165-NP	Nontypeable	L. Bakaletz
86-028NP	Nontypeable	L. Bakaletz
1128MEE	Nontypeable	L. Bakaletz
1728MEE	Nontypeable	L. Bakaletz
AMC 36-A-3	Type a	ATCCc
AMC 36-A-5	Type c	ATCC
AMC 36-A-6	Type d	ATCC
AMC 36-A-7	Type e	ATCC
AMC 36-A-8	Type f	ATCC
E. coli
TOP10F′	Host cloning strain	Invitrogen
DH5α	Host cloning strain	25
Plasmids
pUC18	BamHI- and alkaline phosphatase-treated cloning vector	Pharmacia
pCR2.1	TA cloning vector	Invitrogen
pF3031	24-MDa H. influenzae biogroup aegyptius plasmid	This study

^aAll of the H. influenzae biogroup aegyptius strains listed, except F4380, Connecticut, and Valparaiso, are clinical isolates from Brazil and contain 24-MDa plasmid pF3031.

^bCDC, Centers for Disease Control and Prevention.

^cATCC, American Type Culture Collection.