FIG. 3.

Region of the E2348/69 LEE containing cesF. cesF, encoding the chaperone for the type III secreted effector protein EspF, is located near the map and tir genes, which encode other type III secreted effector proteins, and also near cesT, which encodes the chaperone for tir. However, cesF is distant from and transcriptionally isolated from the LEE4 operon that contains espF. cesF is also transcribed divergently from the adjacent map gene and from orf18, which is part of the LEE3 operon that encodes components of the type III secretion system. An expanded view of cesF shows the EcoRI and BglII sites that were used to construct the cesF mutant strain. The nucleotide (nt) scale is from the LEE sequence described by Elliott et al. (5).