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Western blots of secreted proteins and whole bacteria probed with antiserum against EspF. Mutation of cesF does not affect secretion of EspABD or Tir, and the secretion of EspF by the cesF mutant is only slightly less than that by the wild type. In contrast, the EspF levels in whole bacteria are substantially lower in a cesF mutant. EspF levels are restored by complementation with cloned CesF.
