TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant genotype or description | Reference |
|---|---|---|
| Strains | ||
| E2348/69 | Wild-type E. coli O127::H7 EPEC | 18 |
| E2348/69 cesF | E. coli E2348/69 cesF::pSE887 | This study |
| E2348/69 escN | Type III secretion-deficient mutant | 12 |
| E2348/69 ΔespF | espF deletion | 22 |
| Plasmids | ||
| pCesF-F | pBluescript KS::PlacZ-cesF | This study |
| pCesF-R | pBluescript KS::cesF-PlacZ | This study |
| pQE30::His6CesF | N-terminal MRGSHis6 fusion to CesF | This study |
| pJP5603 | oriR6K mobRP4 lacZ::MCS kan | 29 |
| pBPM37 | EspF-CyaA fusion | 23 |
| pSE887 | pJP5603::cesF internal fragment | This study |