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. 2002 May;70(5):2519–2525. doi: 10.1128/IAI.70.5.2519-2525.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 1. — Total hemolytic activities of mouse serum complement. Well 1, quality control. Wells 2 to 8, dilutions of the standard using the manufacturer's standard. Wells 9 to 11, serum from WT mice, showing positive reactions (complement is present). Similar positive reactions were obtained with serum from C3^+/+, C5^+/+ CR2^+/+, and CR2^−/− mice (not shown). Wells 12 to 14, serum from C5^−/− mice, showing no reaction (complement is absent). Similar negative reactions were obtained with serum from CVF-pretreated WT, C3^+/+, and C5^+/+ mice (not shown).