FIG. 5.

In vitro activity of the K10 MAb on A. fumigatus conidial germination. Swollen A. fumigatus conidia (5 × 10⁴) were incubated at 37°C with 12.5 μg of the K10 MAb in 1 ml of Sabouraud broth. At selected time points, percent germination was calculated and photomicrographs (original magnification, ×40) were taken. A total of 100 conidia per field were counted at a magnification of ×400, and the mean value of three independent counts was calculated. The MTT reduction assay was employed to assess viability of hyphae germinated from Aspergillus conidia after 4 h of exposure to the K10 MAb (A and B). Note the differences in MTT reduction between K10 MAb-treated and untreated cultures. The data reported are from one representative experiment out of three experiments with similar results. ∗, P < 0.05 (K10 MAb-exposed conidia versus control MAb-exposed conidia) according to Student's t test.