FIG. 1.

Total body weights and liver and spleen weights (A) and bacterial counts in livers, spleens, and lymph nodes (B) of C3H/HeN mice infected with S. enterica serovar Typhimurium 14028s (open circles and white bars) and DLG294 (black squares and black bars) at several time points after infection. (C) Bacterial counts of C3H/HeN mice infected with S. enterica serovar Typhimurium 14028s (white bars) and DLG294 complemented by the low-copy-number plasmid pWSK29 carrying sspJ (DLG294-pTS175; checkered bars) on day 6 after infection. C3H/HeN mice were infected subcutaneously in the flanks with ∼30,000 CFU of S. enterica serovar Typhimurium 14028s, DLG294, or DLG294-pTS175. The actual dosage was confirmed by plating serial dilutions of the inoculum. At the indicated time points, livers, spleens, and lymph nodes were aseptically removed and weighed, and the viable counts within these organs were determined by plating serial dilutions (n = 4 per group). The results are expressed as log₁₀ viable counts (means ± standard errors of the means). Asterisks indicate that the number of bacteria of wild-type 14028s is significantly different from that of DLG294 (Student's t test).