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Demonstration of common TCRs in PBMC stimulated with hsp's and gingival tissue from the periodontitis patient. PCR products encoding TCR Vβ genes from PBMC and gingival tissue were analyzed by the SSCP method as described in Materials and Methods. Arrowheads indicate identical clones. Tissue, gingival tissue-derived T-cell clones; Hsp60, hsp60-stimulated PBMC; Pg GroEL, P. gingivalis GroEL-stimulated PBMC.
