TABLE 2.
Amino acid sequences of the common TCR Vβ genes from T cells in gingival tissues and hsp60- and P. gingivalis GroEL-stimulated PBMC in periodontitis patients
| Patient (Vβ family) and sample | Amino acid sequencea
|
Jβ | Frequency | |
|---|---|---|---|---|
| Vβ | N-D-N | |||
| P1 (Vβ 16) | ||||
| Gingiva | CASS | RTLQ | 2.5 | 4/17 |
| hsp60-stimulated PBMC | CASS | RTLQ | 2.5 | 3/17 |
| P3 (Vβ 13.1) | ||||
| Gingiva | CASS | YSTGTGS | 1.1 | 4/7 |
| hsp60-stimulated PBMC | CASS | YSTGTGS | 1.1 | 3/11 |
| P5 (Vβ 9) | ||||
| Gingiva | CASS | QERLAGPP | 2.2 | 3/4 |
| hsp60-stimulated PBMC | CASS | QERLAGPP | 2.2 | 2/6 |
| P. gingivalis GroEL-stimulated PBMC | CASS | QERLAGPP | 2.2 | 3/18 |
| P6 (Vβ 6) | ||||
| Gingiva | CASS | SGLNR | 2.7 | 4/5 |
| hsp60-stimulated PBMC | CASS | SGLNR | 2.7 | 3/4 |
| P8 (Vβ 16) | ||||
| Gingiva | CASS | HARD | 2.4 | 7/33 |
| hsp60-stimulated PBMC | CASS | HARD | 2.4 | 2/20 |
| P. gingivalis GroEL-stimulated PBMC | CASS | HARD | 2.4 | 2/24 |
| P9 (Vβ 8) | ||||
| Gingiva | CASS | APGTAF | 1.5 | 3/28 |
| P. gingivalis GroEL-stimulated PBMC | CASS | APGTAF | 1.5 | 3/30 |
| P9 (Vβ 8) | ||||
| Gingiva | CA | IRPS | 2.3 | 2/28 |
| hsp60-stimulated PBMC | CASS | RPSG | 2.7 | 4/24 |
| P. gingivalis GroEL-stimulated PBMC | CASS | RPSG | 2.7 | 2/30 |
a
Junctional sequences of TCR Vβ genes, which were commonly found in gingival tissues and hsp60-stimulated and P. gingivalis GroEL-stimulated PBMC from patients whose SSCP gels are presented in Fig. 5.