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. 2002 May;70(5):2399–2407. doi: 10.1128/IAI.70.5.2399-2407.2002

FIG. 1.

FIG. 1.

SDS-PAGE of S. aureus cell wall extracts. (A) Clinical and laboratory strains grown in iron-rich (lanes 1, 3, and 5) and iron-restricted (lanes 2, 4, and 6) CRPMI showing major iron-regulated proteins of 40 and 87 kDa. Lanes: 1 and 2, BB; 3 and 4, 8325-4; 5 and 6, RN6390-B. (B) RN6390-B grown in iron-rich and iron-restricted CRPMI treated with proteinase K showing the surface exposure of the 40- and 87-kDa proteins. Lanes: 1, CRPMI; 2, CRPMI plus proteinase K; 3, iron-rich CRPMI; 4, iron-rich CRPMI plus proteinase K. (C) Fresh clinical isolates grown in CRPMI showing conservation of the 40- and 87-kDa proteins and demonstrating the size variation of both proteins. (D) Differential expression of 40- and 87-kDa proteins. Lanes: 1, RN6390-B recovered from chambers without subculture in vitro; 2 to 5, RN6390-B grown in CRPMI or TSM (lanes 2 and 4) or iron-rich CRPMI or TSM (lanes 3 and 5). (E) Immunoblot of RN6390-B cell wall extracts with pooled human serum. Lanes: 1, cells grown in vivo; 2, CRPMI; 3, iron-rich CRPMI; 4, TSM; 5, iron-rich TSM; 6, LB. The 87- and 40-kDa proteins are indicated. M, size marker.