FIG. 6.
Influence of human plasma on cytokine production by DC infected with N. meningitidis. DC (5 × 105 per well) were infected with wild-type (WT) strains A2044, H44/76, and C1701 and their isogenic capsule-deficient mutant strains. DC were infected at an MOI of 1 in RPMI 1640 medium containing 1% heat-inactivated autologous human plasma (+) or in RPMI 1640 medium without plasma (−). At 6 h p.i., DC supernatants were sampled and assessed for TNF-α (A), IL-6 (B), and IL-8 (C) by ELISA. Error bars indicate standard deviations.