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. 2002 May;70(5):2441–2453. doi: 10.1128/IAI.70.5.2441-2453.2002

FIG. 5.

FIG. 5.

RFLP analysis of the virulence regions of the various strains. (a) EcoRI-digested genomic DNAs were probed with ctxAB gene probes (upper panel) and the ctx core probe (lower panel). EcoRI does not cut within the CTXφ genome, and each hybridizing band therefore represents one copy of CTX present in a strain. (b) SphI-digested genomic DNAs of the strains were probed with five probes spanning a 45-kb region surrounding the CTXφ genome integrated on chromosome I. The probes and the sizes of the fragments and the corresponding bands are as follows: ctxrgn 1, 1,106 and 13,818 bp (bands a and j); ctxrgn 2, 2,862 and 13,387 bp (bands e and b); ctxrgn 3, 3,255 and 1,365 bp (bands d and i); ctxrgn 4, 2,651 and 2,180 bp (bands f and h); and ctxrgn 5, 2,651 and 11,400 bp (bands g and c). (c) Genomic DNAs digested with XmnI and probed simultaneously with five different probes from the VPI region. The identities of the bands were confirmed by separate hybridization with individual probes. The probes and the sizes of the fragments and the corresponding bands are as follows: ald, 11,064 bp (band b); tagD, 3,994 bp (band e) and 837 bp (band h); tcpAdn, 3,273 bp (band f) and 1,070 bp (band g); toxT, 12,332 bp (band a); and vpi0845, 3,976 bp (band d) and 7,685 bp (band c).