TABLE 2.
Summary of results from SEM visualization of gallstones
| Prepn | Appearance of bacteria on gallstones after:
|
|
|---|---|---|
| 4 Days | 14 Days | |
| S. enterica serovar Typhimurium, LB broth | No bacteria present | No bacteria present |
| S. enterica serovar Typhimurium, LB broth + 3% bile | No bacteria present | Bacteria (biofilm) present |
| S. enterica serovar Typhi, LB broth | No bacteria present | No bacteria present |
| S. enterica serovar Typhi, LB broth + 3% bile | No bacteria present | Bacteria (biofilm) present |
| S. enterica serovar Typhi, capsular mutant (ViAg−) | No bacteria present | Bacteria (biofilm) present |
| S. enterica serovar Typhimurium flagellar mutant (FliA−) | No bacteria present | Bacteria present, altered appearance |
| S. enterica serovar Typhimurium fimbrial mutants (Fim−, Pef−, Agf−, Lpf−)a | Bacteria (biofilm) present | Bacteria (biofilm) present |
| S. enterica serovar Typhimurium quorum sensing mutant (LuxS−) | No bacteria present | Few bacteria present |
| S. enterica serovar Typhimurium rough LPS mutant (GalE−) | No bacteria present | Bacteria present, altered appearance |
a
The background strain for these S. enterica serovar Typhimurium mutants is SR11; the background strain for all other S. enterica serovar Typhimurium mutants is ATCC 14028s. Biofilm formation on gallstones by wild-type strain SR11 is indistinguishable from biofilm formation on gallstones by the SR11 fimbrial mutants.