FIG. 1.

Western blot analysis of colostrum. Colostrum samples from an intimin-vaccinated dam (lanes 1 and 2) and a sham (TBS)-vaccinated dam (lanes 3 and 4) were assayed by Western blotting. (A) Reactivity of colostrum samples against whole-cell lysates of EHEC O157: H7 strain 86-24 (lanes 1 and 3) and intimin mutant strain 86-24 eaeΔ10 (lanes 2 and 4). The region of the blot containing intimin (arrow) is shown; the colostrum also reacted with lower-molecular-weight bands in the lysates. The band migrating just below intimin may be a cross-reacting band. (B) Reactivity of colostrum samples with a purified N-terminal two-thirds fragment of intimin (lanes 1 and 3) and a purified C-terminal one-third fragment of intimin (lanes 2 and 4) from EHEC strain 86-24.