TABLE 2.
Peptide sequences, derived by ESI-MS, of the novel leptospiral OMPs identified during this study
| Protein | Amino acid sequence(s)a |
|---|---|
| pL18 | DGE[L/I]VT[F/M]GK, YE[L/I]QSH[L/I]ER |
| pL21 | TASV[L/I]VSQSQGVVK |
| pL22 | ST[L/I]QT[L/I]VYGK |
| pL24 | [L/I]SPYDEFA[L/I], PFDTNNTAE[L/I] [L/I]SNFAK |
| pL45 | [F/M]TG[L/I]NADEATK, YNTAATGTYK |
| pL47/49 | VETESVA[L/I]APHR, TVETSTPVA[L/I]TAQK |
| pL50 | PDAAVTY[L/I]FQG[L/I]GK |
| pL55 | VV[F/M]AAETVNH[L/I]R, AV[L/I][L/I]QGTR, [L/I]GDEDHET |
a
Both isoleucine and leucine have the same mass, leaving an ambiguity in the peptide sequence (indicated by [L/I]). Phenylalanine and oxidized methionine have the same mass, which also leaves an ambiguity in the peptide sequence (indicated by [F/M]).