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. 2002 May;70(5):2650–2656. doi: 10.1128/IAI.70.5.2650-2656.2002

FIG. 4.

FIG. 4.

(A) Urinary THP stimulates IL-8 expression in granulocytes. Shown is the effect of THP dose (10 ng/ml to 10 μg/ml) on IL-8 expression by human granulocytes. Granulocytes were incubated with various concentrations of THP as indicated or LPS (1 μg/ml) for 24 h, and IL-8 expression was detected by ELISA (n = 3 determinations/bar). In the presence of anti-THP (right bar) the effect of THP (1 μg/ml) was reduced to control levels. (B) Stimulation of IL-8 by apical versus basal supernatants of MDCK:THP+ and MDCK:THP− cells. MDCK:THP+ and MDCK:THP− cells were cultured on polycarbonate microtiter plates, and the apical and basal cell culture media were harvested separately. IL-8 expression by human granulocytes was measured by ELISA as described in Materials and Methods. Control granulocytes were incubated with cell culture medium only. Bars show means of six determinations/column plus standard deviations. ∗, P < 0.05 versus value for THP+ basal medium; ∗∗, P < 0.01 versus value for controls or MDCK:THP− apical cell supernatants (U test).