Serum IgG antibody response in B10.BR mice immunized parenterally with the LCP-J8 construct in the presence and absence of CFA for exp 1 (A) and exp 2 (B). Antibody titers to the J8 peptide for individual mice are shown, with the average titers (geometric means) represented as bars. Antibody titers to J8 are shown for individual mice from the control group that had been immunized with J8 in CFA. The two experiments were performed separately and differ in that mice received four booster injections of immunogen in exp 1 and five booster injections in exp 2. Using analysis of variance (with Tukey's post hoc method for multiple comparisons), antibody titers were significantly greater in mice immunized with LCP-J8/CFA than in those immunized with J8 in CFA alone (exp 1; ∗, P < 0.001). Significant differences in antibody titers were also observed as indicated (∗∗, P < 0.001; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.001).