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. 2002 May;70(5):2734–2738. doi: 10.1128/IAI.70.5.2734-2738.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 3. — Opsonization of GAS by LCP-J8/CFA, LCP-J8/PBS, and control (J8/CFA and pepM1/CFA) B10.BR mouse antisera for exp 1 (panels A and C to E) and exp 2 (B). The average percentage of opsonization (measured as the percentage of reduction in bacterial population [in CFU]) for each individual group is shown with the standard error of the mean represented as a bar. Opsonization was determined by an indirect bactericidal assay which compares the growth of bacterial population (in CFU) following incubation in the presence of immune sera to that of control normal mouse sera obtained from mice immunized with adjuvant in PBS alone. Using analysis of variance (with Tukey's post hoc method for multiple comparisons), opsonization against M1 and BSB24 GAS was significantly greater in mice immunized with LCP-J8/CFA and LCP-J8/PBS than in those immunized with J8 in CFA alone. ∗, P = 0.051; ∗∗, P = 0.008; ∗∗∗, P = 0.031; ∗∗∗∗, P = 0.013.

FIG. 3. — Opsonization of GAS by LCP-J8/CFA, LCP-J8/PBS, and control (J8/CFA and pepM1/CFA) B10.BR mouse antisera for exp 1 (panels A and C to E) and exp 2 (B). The average percentage of opsonization (measured as the percentage of reduction in bacterial population [in CFU]) for each individual group is shown with the standard error of the mean represented as a bar. Opsonization was determined by an indirect bactericidal assay which compares the growth of bacterial population (in CFU) following incubation in the presence of immune sera to that of control normal mouse sera obtained from mice immunized with adjuvant in PBS alone. Using analysis of variance (with Tukey's post hoc method for multiple comparisons), opsonization against M1 and BSB24 GAS was significantly greater in mice immunized with LCP-J8/CFA and LCP-J8/PBS than in those immunized with J8 in CFA alone. ∗, P = 0.051; ∗∗, P = 0.008; ∗∗∗, P = 0.031; ∗∗∗∗, P = 0.013.