cnfY probe hybridization to a Southern blot of chromosomal DNAs from Y. pseudotuberculosis strains. Lane 1, YPIII; lane 2, YPIIIc; lane 3, 713425; lane 4, 722080; lane 5, 730317; lane 6, 730440-1; lane 7, ATCC 29833. One microgram of each DNA was digested with NsiI. The numbers in the left margin indicate molecular size standards in kilobases. The autoradiogram was scanned, and the digital image file was annotated with Canvas.