FIG. 2.

Blood, livers, and spleens of F5 mice on a C57BL/6 background were infected with 4.1 × 10⁵ Listeria organisms intravenously and harvested at 72 h. Serial dilutions of blood or ground tissues were plated on LB agar plates. The CFU in 30 μl of blood (A) or the log₁₀ CFU (adjusted for total organ weight) in liver and spleen (B) were determined for Cas11^−/− mice (n = 11) and wild-type controls (n = 4). Mean values from individual mice (± standard errors of the means) are shown. There was no significant difference for liver, spleen, and blood as determined by the unpaired Student t test.