FIG. 2.

Verification of the presence of the type 1 wav gene cluster in four different strains by PCR analysis. (A) Order and orientation of the wav genes and calculated theoretical length of PCR products 1 to 19. The primers used were ORF-specific sense or antisense oligonucleotides (Table 2). (B) Ethidium bromide stained-agarose gel showing the electrophoretic mobilities of PCR products 1 to 19 obtained from strains V243 (lanes A) (O1 environmental isolate), V244 (lanes B) (O37 clinical isolate), P27459 (lanes C) (O1 El Tor pandemic isolate); and MO10 (lanes D) (O139 epidemic isolate). Lanes S, molecular size standard.