TABLE 2.
Primers used for wav gene amplification
| ORF | Type or straina | Primer (5′→3′)
|
bp | |
|---|---|---|---|---|
| Sense | Antisense | |||
| wavA | 1-5 | GGCAAAATCAACTAACAATCCGGb | GGCGCAAGCTCAGTCAATAC | 1,396 |
| wavB | 1-5 | wavBintSalI | wavBintEcoRI | 538 |
| waaC | 1-5 | TCCCTCTGTATCCTGCGTTT | ACCTGTGTGGCCATGATGT | 1,031 |
| wavC | 1 | CTGCAAAACTACCGGATAACGc | AGCAAACGCTTCAAGACTCC | 713 |
| wavC | 1-5 | CTGCAAAACTACCGGATAACG | TCCATACCTTCTCTTGGTCA | 582 |
| wavD | 1 | ACACCGTCCATTACCTCCAC | TTCTTAATTTTAGCCTAACTCCTTTCC | 880 |
| wavE | 1-4 | TTGATGATGAAGCGATCACC | GCAGCCAATCCTTAAGGTCA | 816 |
| wavF | 1-4 | GGGAATGAGTTCTCGCTTCTT | GGCTGCCTCAAAAAGTCAGT | 714 |
| wavG | 1-4 | ATGTCTGGTGCCTATGTTGG | AAAGGCAACATGGAGAGGAA | 1,487 |
| wavH | 1-4 | GGCACTCTCACCCAAGCTAA | AAATTCGGAAGGGCGCACGG | 294 |
| waaA | 1-4 | GTATGGCCTCTATCGCCGTA | TTTTCGAGAGCTCCACGATT | 1,182 |
| waaA | 5 | TCCGTGGCCTCTATACCTTG | TTTTGGATAGAACCAGACGATTC | 1,237 |
| wavI | 1-4 | GTATTGATCGCCAACCGACT | CGAGATCTTCGGGATTGATG | 757 |
| wavI | 5 | TGATTCGTCTGACTGGCAAC | TTTGAACGAGGGCGATCTAT | 712 |
| wavJd | 1 | CATGAAACACCTCTGGTTTG | CGAAAGGGAACCGTAGCATA | 912 |
| waaF | 1 | waaFPstI | waaFFspI | 1,114 |
| waaF | 2-5 | TGATTGTAGGCCCTTCTTGG | TAGCGCTTCAATGACACGAG | 1,004 |
| waaL | 1 and 2 | waaLNheI | waaLSalI | 1,295 |
| waaL | V194 | TTTCTCCACTAATTGTTCTGCTG | TACAGGACTACTTGAATCAC | 743 |
| waaL | 5 | TGGAATAAACATCGGCATTACA | CATAGCGCCAACAAAGAGAA | 1,104 |
| wavK | 1 and 2 | ATGGCTGGCTTTGGTTTAAT | AATGCAATACTGGCCAATCG | 495 |
| wavL | 1-4 | ATGAATATTTTGATGGCCCT | ATTAAACCAAAGCCAGCCAT | 1,806 |
| wavL | 5 | TTCTAATGGCCCTATCCCAAC | TCTTGGAAAAATCGCAATCC | 1,773 |
| gmhDd | 1-5 | ATGATTATCGTAACTGGCGG | TTACTTACGATTAATCAGCG | 944 |
| wavM | V194 | TGCATCAATTGAACAGCAAA | TAGATTTCTCGCGTCGGTTT | 630 |
| wavN | 4 | GATTGGATCCCTGGAGGATT | TGCTAATACTCCCGCACCTT | 1,881 |
| wavNO | 4 | CCAAAAACGCTTTTTAACTG | GATTGGATCCCTGGAGGATT | 2,800 |
| wavP | 5 | GATGTCGAGGCATTAAACTCG | ATTGGTCGATCCCCTTCTTG | 769 |
| wavQ | 5 | GCAAAAATGGTGGGGTAGTT | ACACACTCTTCTGAACGACGA | 841 |
| wavR | 5 | TCCCATTTAACAAACCACCA | TTTCTTCCCGCTACTGTGCT | 991 |
| wavS | 5 | ACAGATCGGCGATGTCAGTT | GTTGCGTTGCAACAGATAGG | 545 |
| wavT | 5 | ACACAAATGCGTTGAGATGC | TGCTTCTTTGTAGCCATTGA | 776 |
a
Primers give positive signals for the indicated strain or type of wav gene cluster (Fig. 1).
b
Primer binds in kdtB (VC0222), downstream of wavA.
c
Underlined primers are identical.
d
Annealing at 54°C; all others anneal at 56°C.