FIG. 3.

Tick cell death in IDE8 tick cell cultures infected with B. burgdorferi N40 (1 × 10⁷ to 2 × 10⁷ cells/ml; MOI = 5): mean (± standard error of the mean) percentages of necrotic (A) and apoptotic (B) tick cells in infected (solid lines) and uninfected (dashed lines) tick cell cultures. See Materials and Methods for details. Each point represents the results from 6 to 10 measurements pooled from two independent complete experiments. The differences in the percentages of necrotic cells between infected and uninfected cultures are significant; the differences in the percentages of apoptotic cells between infected and uninfected cultures are not significant (as determined by one-way analysis of variance with the Tukey-Kramer multiple-comparison post test). ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001.