TABLE 1.
Oligonucleotide primers and LightCycler hybridization probes
| Target gene | GenBank accession no. | Primer | Sequencea |
|---|---|---|---|
| gyrB | D10489 | gyrU | TTATGGTGCTGGGCAAATACA |
| gyrL | CACCATGTAAACCACCAGATA | ||
| gyrFL1 | ATTTTAACTGTTTTACATGCTGGTGGTAA-F | ||
| gyrLC1 | Red640-TTTGGCGGTGGCGGATACA-ph | ||
| clfA | Z18852 | clfU | GGCGTGGCTTCAGTGCTTGTA |
| clfL | CACCAGTTACCGGCGTTTCTTC | ||
| clfFL1 | GGCGCAAAATCCAGCACAACAGGAA-F | ||
| clfLC1 | Red640-CGACACAATCATCATCAACAAATGC AAC-ph |
a
F, fluorescein; Red640, LightCycler-Red 640-N-hydroxysuccinimide ester; ph, 3′-phosphate.